An investigation of serum lipids and lipoproteins by paper electrophoresis.

In view of the importance now attached to them it is remarkable that the lipoproteins of human serum received so little attention from early workers. In 1929 Machboeuf isolated an alpha-lipoprotein from horse serum and in 1935 McFarlane, in the course of ultracentrifuge studies of human serum, discovered the " X-Fraction " which was later shown by Pedersen to be beta-lipoprotein. The development of large-scale ethanol fractionation of human plasma pools led to a much better understanding of the lipoproteins, particularly betalipoprotein (Oncley, Gurd, and Melin, 1950). Recently there has been evidence that the lipids